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Product Info Summary
| SKU: | A08379-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ADAM2 Antibody Picoband™
SKU/Catalog Number
A08379-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADAM2 Antibody Picoband™ catalog # A00428-4. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ADAM2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08379-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human ADAM2 recombinant protein (Position: L228-Y277). Human ADAM2 shares 72% amino acid (aa) sequence identity with both mouse and rat ADAM2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08379-2 is reactive to ADAM2 in Human
Applications
A08379-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
69 kDa
Calculated molecular weight
82.457kDa
Background of ADAM2
Disintegrin and metalloproteinase domain-containing protein 2 or Beta-fertilin is an enzyme that in humans is encoded by the ADAM2 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded protein is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg /1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ADAM2 using anti-ADAM2 antibody (A08379-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM2 antigen affinity purified polyclonal antibody (Catalog # A08379-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM2 at approximately 69 kDa. The expected band size for ADAM2 is at 82 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-ADAM2 antibody (A08379-2).
Overlay histogram showing MCF-7 cells stained with A08379-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAM2 Antibody (A08379-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ADAM2 (Source: Uniprot.org, NCBI)
Gene Name
ADAM2
Full Name
Disintegrin and metalloproteinase domain-containing protein 2
Weight
82.457kDa
Alternative Names
ADAM metallopeptidase domain 2; Cancer/testis antigen 15; CRYN2; CT15ADAM 2; disintegrin and metalloproteinase domain-containing protein 2; EC 3.4.24; fertilin beta; Fertilin subunit beta; FTNBCRYN1; PH-30; PH-30b; PH30PH30-beta ADAM2 CRYN1, CRYN2, CT15, FTNB, PH-30b, PH30, PH30-beta ADAM metallopeptidase domain 2 disintegrin and metalloproteinase domain-containing protein 2|cancer/testis antigen 15|fertilin subunit beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAM2, check out the ADAM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAM2 Antibody Picoband™ (A08379-2)
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